Enzymatic and Cryoreduction EPR Studies of the Hydroxylation of Methylated Nω-Hydroxy-l-arginine Analogues by Nitric Oxide Synthase from Geobacillus stearothermophilus
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Enzymatic and Cryoreduction EPR Studies of the Hydroxylation of Methylated Nω-Hydroxy-l-arginine Analogues by Nitric Oxide Synthase from Geobacillus stearothermophilus
Nitric oxide synthase (NOS) catalyzes the conversion of L-arginine to L-citrulline and NO in a two-step process involving the intermediate N(ω)-hydroxy-L-arginine (NHA). It was shown that Cpd I is the oxygenating species for L-arginine; the hydroperoxo ferric intermediate is the reactive intermediate with NHA. Methylation of the N(ω)-OH and N(ω)-H of NHA significantly inhibits the conversion of...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2014
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi500485z